Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions (Q24596251)

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R K Brachmann

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A Fattaey

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E Harlow

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J D Boeke

Proceedings of the National Academy of Sciences of the United States of America

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language of work or name

English

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publication date

17 September 1996

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published in

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volume

93

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issue

19

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page(s)

10315-20

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cites work

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E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle

24 March 2017

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The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit

24 March 2017

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Mammalian Ras interacts directly with the serine/threonine kinase Raf

24 March 2017

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A novel genetic system to detect protein-protein interactions

24 March 2017

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A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae

24 March 2017

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24 March 2017

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A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

24 March 2017

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Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast

24 March 2017

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Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein

24 March 2017

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Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun

27 September 2017

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Definition of a consensus binding site for p53

24 March 2017

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Transcription activation by the adenovirus E1a protein

7 April 2017

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Suppression of human colorectal carcinoma cell growth by wild-type p53

7 April 2017

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The E2F transcription factor is a cellular target for the RB protein

7 April 2017

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Dominant-negative p53 mutations selected in yeast hit cancer hot spots

7 April 2017

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Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae

27 September 2017

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Inhibition of E2F-1 transactivation by direct binding of the retinoblastoma protein

27 September 2017

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A comparison of the biological activities of wild-type and mutant p53.

27 September 2017

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The INO1 promoter of Saccharomyces cerevisiae includes an upstream repressor sequence (URS1) common to a diverse set of yeast genes

27 September 2017

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Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system

27 September 2017

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Independent regions of adenovirus E1A are required for binding to and dissociation of E2F-protein complexes

27 September 2017

1 reference

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Identification of essential nucleotides in an upstream repressing sequence of Saccharomyces cerevisiae by selection for increased expression of TRK2.

27 September 2017

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pRB, p107 and the regulation of the E2F transcription factor

27 September 2017

1 reference

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Lack of cyclin D-Cdk complexes in Rb-negative cells correlates with high levels of p16INK4/MTS1 tumour suppressor gene product

27 September 2017

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Isolation of a cDNA encoding a metal response element binding protein using a novel expression cloning procedure: the one hybrid system

27 September 2017

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Structure-function relationship of yeast S-II in terms of stimulation of RNA polymerase II, arrest relief, and suppression of 6-azauracil sensitivity

27 September 2017

1 reference

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A new version of the two-hybrid assay for detection of protein-protein interactions

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Developmental regulation of SPO13, a gene required for separation of homologous chromosomes at meiosis I

27 September 2017

1 reference

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Nucleotide sequence and promoter analysis of SPO13, a meiosis-specific gene of Saccharomyces cerevisiae

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

A cis-acting element present in multiple genes serves as a repressor protein binding site for the yeast CAR1 gene

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

p53 function and dysfunction

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Adenovirus E1A targets key regulators of cell proliferation

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Overcoming telomeric silencing: a trans-activator competes to establish gene expression in a cell cycle-dependent way.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Presence of a potent transcription activating sequence in the p53 protein

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Transcriptional regulation of a yeast HSP70 gene by heat shock factor and an upstream repression site-binding factor

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Adenovirus E1a prevents the retinoblastoma gene product from complexing with a cellular transcription factor

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Structure and function of the yeast URA3 gene: expression in Escherichia coli.

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=38381

Screening patients for heterozygous p53 mutations using a functional assay in yeast

28 November 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/8816797

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Yeast promoters URA1 and URA3. Examples of positive control

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/8816797

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1073/PNAS.93.19.10315

1 reference

ADS bibcode

1996PNAS...9310315V

0 references

1 reference

1 reference

PubMed publication ID

8816797

1 reference