Current two-dimensional electrophoresis technology for proteomics (Q22252855)

Europe PubMed Central

title

Current two-dimensional electrophoresis technology for proteomics (English)

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1

Angelika Görg

Angelika

Görg

1 reference

PubMed

PubMed publication ID

7 February 2023

Walter Weiss

2

Walter

Weiss

1 reference

PubMed

PubMed publication ID

15543535

7 February 2023

Michael J. Dunn

series ordinal

3

author given names

Michael J

author last names

Dunn

1 reference

PubMed

PubMed publication ID

15543535

Proteome profiling-pitfalls and progress

7 February 2023

language of work or name

English

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publication date

15 November 2004

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published in

Proteomics

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volume

4

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issue

12

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page(s)

3665-3685

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cites work

1 reference

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7 January 2021

Perspectives for mass spectrometry and functional proteomics

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7 January 2021

Quantitative evaluation of protein recoveries in two-dimensional electrophoresis with immobilized pH gradients

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7 January 2021

Organic solvent extraction as a versatile procedure to identify hydrophobic chloroplast membrane proteins

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7 January 2021

A turning point in proteome analysis: sample prefractionation via multicompartment electrolyzers with isoelectric membranes

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7 January 2021

Towards global analysis of mammalian proteomes using sample prefractionation prior to narrow pH range two-dimensional gels and using one-dimensional gels for insoluble and large proteins

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7 January 2021

Zooming-in on the proteome: very narrow-range immobilised pH gradients reveal more protein species and isoforms

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https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Inhibition of unwanted proteolysis during sample preparation: evaluation of its efficiency in challenge experiments

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Use of antibodies for detection of phosphorylated proteins separated by two-dimensional gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

A comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gels

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https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Proceedings of the British Electrophoresis Society Meeting. York, United Kingdom, 4-6 April 2001

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7 January 2021

Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains

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7 January 2021

Comprehensive analysis of complex proteomes using microscale solution isoelectrofocusing prior to narrow pH range two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Differential gel exposure, a new methodology for the two-dimensional comparison of protein samples.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

An improved formulation of SYPRO Ruby protein gel stain: comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Web-based two-dimensional database of Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry

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https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Gradiflow as a prefractionation tool for two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Sample prefractionation with Sephadex isoelectric focusing prior to narrow pH range two-dimensional gels

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in the first-dimensional isoelectric focusing gel with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Isoelectric focusing of basic proteins: the problem of oxidation of cysteines

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

The current state of two-dimensional electrophoresis with immobilized pH gradients

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Extraction of plant proteins for two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

IPGMAKER: a program for IBM-compatible personal computers to create and test recipes for immobilized pH gradients

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Giant two-dimensional gel electrophoresis: Methodological update and comparison with intermediate-format gel systems

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Temperature-dependent spot positional variability in two-dimensional polypeptide patterns

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Immobilized pH gradients (IPG) simulator--an additional step in pH gradient engineering: II. Nonlinear pH gradients

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Application of sequential extraction procedures and glycoprotein blotting for the characterization of the 2-D polypeptide patterns of barley seed proteins

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Electrophoretic characterization of wheat grain allergens from different cultivars involved in bakers' asthma

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

A nonlinear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Micropreparative two-dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: an interlaboratory comparison

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal systems

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two-dimensional gel electrophoresis.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional electrophoresis of membrane proteins: a current challenge for immobilized pH gradients

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Isolation of rat hepatic peroxisomes by means of immune free flow electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Difference gel electrophoresis: a single gel method for detecting changes in protein extracts

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Preparative two-dimensional gel electrophoresis of membrane proteins

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional gel electrophoresis for proteome projects: the effects of protein hydrophobicity and copy number

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Reference map of the low molecular mass proteins of Haemophilus influenzae

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Detection of glycoproteins in polyacrylamide gels and on electroblots using Pro-Q Emerald 488 dye, a fluorescent periodate Schiff-base stain

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Isoelectric focusing in long immobilized pH gradient gels to improve protein separation in proteomic analysis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Application of proteomics technologies in the investigation of the brain

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Evaluation of saturation labelling two-dimensional difference gel electrophoresis fluorescent dyes

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

An efficient solubilization buffer for plant proteins focused in immobilized pH gradients

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

The role of bioinformatics in two-dimensional gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Sample preparation for two-dimensional gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Exploitation of specific properties of trifluoroethanol for extraction and separation of membrane proteins

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Towards a phosphoproteome map of Corynebacterium glutamicum.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Improved Ruthenium II tris (bathophenantroline disulfonate) staining and destaining protocol for a better signal-to-background ratio and improved baseline resolution

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Setting up standards and a reference map for the alkaline proteome of the Gram-positive bacterium Lactococcus lactis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Assessing factors for reliable quantitative proteomics based on two-dimensional gel electrophoresis.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

A novel experimental design for comparative two-dimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Multi-component immunoaffinity subtraction chromatography: an innovative step towards a comprehensive survey of the human plasma proteome

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

High throughput protein characterization by automated reverse-phase chromatography/electrospray tandem mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Advances in protein solubilisation for two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Proteomics and automation

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Extraction of Escherichia coli proteins with organic solvents prior to two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Towards the recovery of hydrophobic proteins on two-dimensional electrophoresis gels

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: wide pH gradients up to pH 12, longer separation distances and simplified procedures

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Proteome analysis of polyacrylamide gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Dual channel imaging of two-dimensional electropherograms in Bacillus subtilis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Isolation and biochemical characterization of organelles from the yeast, Saccharomyces cerevisiae

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to first-dimensional isoelectric focusing: imaging of the fluorescent protein spot patterns using a cooled charge-coupled device

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Purification of Saccharomcyes cerevisiae Mitochondria Devoid of Microsomal and Cytosolic Contaminations

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Analytical techniques for cell fractions. XXII. Two-dimensional analysis of serum and tissue proteins: multiple gradient-slab gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

A method for two-dimensional electrophoresis of proteins from green plant tissues

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Tris-tricine and Tris-borate buffer systems provide better estimates of human mesothelial cell intermediate filament protein molecular weights than the standard Tris-glycine system.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

General concepts about cell sorting techniques

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

The use of continuous preparative free-flow electrophoresis for dissociating cell fractions and isolation of membranous components

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polycrylamide layers

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Fluorescent two-dimensional difference gel electrophoresis unveils the potential of gel-based proteomics.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Protein phosphorylation: technologies for the identification of phosphoamino acids.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Enrichment of low-copy-number gene products by hydrophobic interaction chromatography

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Subcellular fractionation, electromigration analysis and mapping of organelles

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

2D protein electrophoresis: can it be perfected?

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

2D or not 2D. Two-dimensional gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional gel electrophoresis: recent advances in sample preparation, detection and quantitation

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Tackling the phosphoproteome: tools and strategies

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

An evaluation of the use of two-dimensional gel electrophoresis in proteomics

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Detection technologies in proteome analysis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Detecting proteins separated by 2-D gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

An integrated microfluidics-tandem mass spectrometry system for automated protein analysis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

ProFound: an expert system for protein identification using mass spectrometric peptide mapping information

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Carbamylation of proteins in 2-D electrophoresis--myth or reality?

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Direct analysis of protein complexes using mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Large-scale analysis of the yeast proteome by multidimensional protein identification technology

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Mass spectrometry-based proteomics

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Proteomic analysis of post-translational modifications

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents: the yeast salinity stress response

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

PNA openers as a tool for direct quantification of specific targets in duplex DNA.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Quantitative Double-Label Radiography of Two-Dimensional Protein Gels Using Color Negative Film and Computer Analysis

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Instability of pH gradients formed by isoelectric focusing in polyacrylamide gel.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Small-scale fractionation of proteins and nucleic acids by isoelectric focusing in polyacrylamide gels

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Laser capture microdissection

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Molecular biologist's guide to proteomics

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Organelle proteomics: implications for subcellular fractionation in proteomics.

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021

Exploring the phosphoproteome with mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FPMIC.200401031

7 January 2021