Making the most of fusion tags technology in structural characterization of membrane proteins (Q37387095)

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scientific article published on 07 February 2009

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English	Making the most of fusion tags technology in structural characterization of membrane proteins	scientific article published on 07 February 2009

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

review article

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Europe PubMed Central

title

Making the most of fusion tags technology in structural characterization of membrane proteins (English)

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

main subject

membrane protein

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author name string

Hao Xie

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1

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

Xiao-Ming Guo

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2

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

Hong Chen

series ordinal

3

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

language of work or name

English

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publication date

7 February 2009

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

published in

Molecular Biotechnology

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

volume

42

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

issue

2

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

page(s)

135-145

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Europe PubMed Central

PubMed publication ID

19199085

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20 August 2017

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Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein

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Structure and mechanism of the lactose permease of Escherichia coli

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A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae

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Membrane topology and insertion of membrane proteins: search for topogenic signals

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Use of gene fusions to study outer membrane protein localization in Escherichia coli

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Analysis of the topology of Vibrio cholerae NorM and identification of amino acid residues involved in norfloxacin resistance

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Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

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Heterodimerization of type II phytochromes in Arabidopsis

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Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis

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A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells

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12 December 2020

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Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli

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A modified mammalian tandem affinity purification procedure to prepare functional polycystin-2 channel

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Insertional gene fusion technology

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12 December 2020

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Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localization

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Techniques and applications of NMR to membrane proteins

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Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor

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12 December 2020

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Mapping the membrane topology and extracellular ligand binding domains of the retinol binding protein receptor

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12 December 2020

based on heuristic

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Estimation of membrane proteins in the human proteome.

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12 December 2020

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Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

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Making the most of affinity tags

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Folding and assembly of bacterial alkaline phosphatase in vitro and in vivo.

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A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes

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PubMed

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The green fluorescent protein

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PubMed

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12 December 2020

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Direct Interaction of Subunits a and b of the F0 Complex of Escherichia coli ATP Synthase by Forming an ab 2 Subcomplex

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PubMed

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Expression and purification of two hydrophobic double-spanning membrane proteins derived from the cystic fibrosis transmembrane conductance regulator.

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

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Membrane topology of the DrrB protein of the doxorubicin transporter of Streptomyces peucetius

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

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Subunit composition of mammalian transient receptor potential channels in living cells

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12 December 2020

based on heuristic

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Crystallisation of membrane proteins mediated by antibody fragments

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based on heuristic

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Epitope tagging

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based on heuristic

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The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/19199085

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Multiple epitope tagging of expressed proteins for enhanced detection

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PubMed

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Retinoblastoma protein purification and transduction of retina and retinoblastoma cells using improved alphavirus vectors

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12 December 2020

based on heuristic

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Gi/o proteins: expression for direct activation enquiry

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

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Purification and initiation of structural characterization of human peripheral myelin protein 22, an integral membrane protein linked to peripheral neuropathies

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PubMed

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based on heuristic

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Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms

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PubMed

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Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins

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PubMed

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based on heuristic

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Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

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Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection.

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PubMed

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The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo

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PubMed

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Escherichia coli fusion carrier proteins act as solubilizing agents for recombinant uncoupling protein 1 through interactions with GroEL.

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Localisation of the PsbH subunit in photosystem II: a new approach using labelling of His-tags with a Ni(2+)-NTA gold cluster and single particle analysis

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PubMed

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Addressing membrane protein topology using the fluorescence protease protection (FPP) assay

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PubMed

reference URL

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A topological model for the general aromatic amino acid permease, AroP, of Escherichia coli

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PubMed

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One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of Strep-tag affinity chromatography

1 reference

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Production and comprehensive quality control of recombinant human Interleukin-1beta: a case study for a process development strategy

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AFM imaging reveals the tetrameric structure of the TRPC1 channel

1 reference

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PubMed

reference URL

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12 December 2020

based on heuristic

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Membrane topology of the electrogenic aspartate-alanine antiporter AspT of Tetragenococcus halophilus

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PubMed

reference URL

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based on heuristic

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Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast

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PubMed

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based on heuristic

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Purification of recombinant proteins by chemical removal of the affinity tag

1 reference

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PubMed

reference URL

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12 December 2020

based on heuristic

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Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins.

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PubMed

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12 December 2020

based on heuristic

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Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Two-dimensional crystallization of Escherichia coli lactose permease

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Fusion protein approach to improve the crystal quality of cytochrome bo(3) ubiquinol oxidase from Escherichia coli

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Peptide tags for labeling membrane proteins in live cells with multiple fluorophores

1 reference

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PubMed

reference URL

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12 December 2020

based on heuristic

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Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli

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12 December 2020

based on heuristic

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Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70

1 reference

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PubMed

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12 December 2020

based on heuristic

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Membrane protein crystallization

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

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Strategy for selecting and characterizing linker peptides for CBM9-tagged fusion proteins expressed in Escherichia coli

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The fluorescence protease protection (FPP) assay to determine protein localization and membrane topology

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Membrane-protein topology

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Recombinant protein expression in Escherichia coli

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Influenza B virus BM2 protein is an oligomeric integral membrane protein expressed at the cell surface.

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A fusion protein system for the recombinant production of short disulfide-containing peptides.

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Analyzing protein complexes in Drosophila with tandem affinity purification-mass spectrometry.

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Epitope tagging

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expression, purification, and membrane reconstitution of a CD4 fragment comprising the transmembrane and cytoplasmic domains of the receptor

1 reference

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expression and characterization of full-length human heme oxygenase-1: the presence of intact membrane-binding region leads to increased binding affinity for NADPH cytochrome P450 reductase

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PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/19199085

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12 December 2020

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Identifiers

DOI

10.1007/S12033-009-9148-X

1 reference

stated in

Europe PubMed Central

PubMed publication ID

19199085

retrieved

20 August 2017

PubMed publication ID

19199085

1 reference

stated in

Europe PubMed Central

PubMed publication ID

19199085

retrieved

20 August 2017

 

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