Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases (Q38810667)

27087594

6 September 2017

title

Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases (English)

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6 September 2017

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J Keith Joung

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

3 June 2020

author name string

Shengdar Q Tsai

series ordinal

1

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

3 June 2020

language of work or name

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1 April 2016

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27087594

6 September 2017

published in

Nature Reviews Genetics

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

3 June 2020

volume

17

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

3 June 2020

issue

5

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

3 June 2020

page(s)

300-312

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

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Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

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Delivery and therapeutic applications of gene editing technologies ZFNs, TALENs, and CRISPR/Cas9.

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Minimizing off-Target Mutagenesis Risks Caused by Programmable Nucleases

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Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery

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One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering

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Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

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Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection.

21 January 2018

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High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

21 January 2018

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High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

21 January 2018

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DNA targeting specificity of RNA-guided Cas9 nucleases

21 January 2018

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CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity

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CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

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The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

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Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

21 January 2018

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E-CRISP: fast CRISPR target site identification

21 January 2018

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Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection

21 January 2018

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Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity

21 January 2018

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In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites

21 January 2018

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Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs

21 January 2018

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Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.

21 January 2018

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Off-target mutations are rare in Cas9-modified mice.

21 January 2018

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An unbiased genome-wide analysis of zinc-finger nuclease specificity

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Evaluation of TCR Gene Editing Achieved by TALENs, CRISPR/Cas9, and megaTAL Nucleases

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Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors.

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GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

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Genome-wide translocation sequencing reveals mechanisms of chromosome breaks and rearrangements in B cells

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Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases.

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Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

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Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

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Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

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Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

21 January 2018

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Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing

21 January 2018

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Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9

21 January 2018

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Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

21 January 2018

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High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.

21 January 2018

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https://api.crossref.org/works/10.1038%2FNRG.2016.28

Rationally engineered Cas9 nucleases with improved specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

21 January 2018

1 reference

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Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

21 January 2018

1 reference

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Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

21 January 2018

1 reference

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Targeted gene knockout by direct delivery of zinc-finger nuclease proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Rational design of a split-Cas9 enzyme complex

21 January 2018

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A split-Cas9 architecture for inducible genome editing and transcription modulation

21 January 2018

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Photoactivatable CRISPR-Cas9 for optogenetic genome editing

21 January 2018

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https://api.crossref.org/works/10.1038%2FNRG.2016.28

Small molecule-triggered Cas9 protein with improved genome-editing specificity

21 January 2018

1 reference

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STRUCTURAL BIOLOGY. A Cas9-guide RNA complex preorganized for target DNA recognition

21 January 2018

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Dynamics of CRISPR-Cas9 genome interrogation in living cells

21 January 2018

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DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

21 January 2018

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Engineered CRISPR-Cas9 nucleases with altered PAM specificities

21 January 2018

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Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

21 January 2018

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Structure and Engineering of Francisella novicida Cas9.

21 January 2018

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DNA-binding-domain fusions enhance the targeting range and precision of Cas9.

21 January 2018

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Conformational control of DNA target cleavage by CRISPR-Cas9.

21 January 2018

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Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

21 January 2018

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Cas9 gRNA engineering for genome editing, activation and repression.

21 January 2018

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Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

21 January 2018

1 reference

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Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture.

21 January 2018

1 reference

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Targetted correction of a mutant HPRT gene in mouse embryonic stem cells

21 January 2018

1 reference

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What's changed with genome editing?

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2016.28

Keeping CRISPR/Cas on-Target

31 March 2020

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/27087594

12 December 2020

inferred from PubMed ID database lookup

Genome editing with engineered zinc finger nucleases

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/27087594

12 December 2020

inferred from PubMed ID database lookup

Open-source guideseq software for analysis of GUIDE-seq data.

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/27087594

12 December 2020

inferred from PubMed ID database lookup

Identifiers

DOI

10.1038/NRG.2016.28

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

Dimensions Publication ID

3 June 2020

1010657174

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Fatcat ID

release_u2kedlgxxvfybdbwulv4vunsru

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/u2kedlgxxvfybdbwulv4vunsru

24 November 2022

mapped directly with Wikidata item

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27087594%20AND%20SRC:MED&resulttype=core&format=json

3 June 2020

27087594

1 reference