Multicolor laser scanning confocal immunofluorescence microscopy: practical application and limitations. (Q30877978)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

review article

1 reference

title

Multicolor laser scanning confocal immunofluorescence microscopy: practical application and limitations (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

immunofluorescence microscopy

16 December 2019

main subject

1 reference

T Clark Brelje

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

Martin W Wessendorf

series ordinal

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

Robert L Sorenson

series ordinal

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

publication date

1 January 2002

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

published in

Methods in Cell Biology

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

volume

70

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

page(s)

165-244

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

Detection of lymphocyte subsets using three-color/single-laser flow cytometry and the fluorescent dye Peridinin chlorophyll-a protein

16 December 2019

cites work

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Results obtained with a sensitive confocal scanning system designed for epifluorescence

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Probing DNA structure and function with a multi-wavelength fluorescence confocal laser microscope.

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Autofluorescence of viable cultured mammalian cells

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Cellular autofluorescence--is it due to flavins?

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF) I. Preparation and fractionation of labelled IgG

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Role of prolactin versus growth hormone on islet B-cell proliferation in vitro: implications for pregnancy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Three-dimensional imaging of intact isolated islets of Langerhans with confocal microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Three-dimensional reconstruction of transmitter-identified central neurons by "en bloc" immunofluorescence histochemistry and confocal scanning microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluorescence quenching of fluorescein by R-phycoerythrin. A pitfall in dual fluorescence analysis

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluorescent protein-dye conjugates. II. Gamma globulin conjugated with various dyes

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Five-parameter fluorescence imaging: wound healing of living Swiss 3T3 cells

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Two- and three-color immunofluorescence using aminocoumarin, fluorescein, and phycoerythrin-labelled antibodies and single laser flow cytometry

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Two-photon laser scanning fluorescence microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Prominent expression of acidic fibroblast growth factor in motor and sensory neurons

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

High-resolution immunogold localization of Giardia cyst wall antigens using field emission SEM with secondary and backscatter electron imaging

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Single laser flow cytometric detection of lymphocytes binding three antibodies labelled with fluorescein, phycoerythrin and a novel tandem fluorochrome conjugate

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Five subtypes of type A gamma-aminobutyric acid receptors identified in neurons by double and triple immunofluorescence staining with subunit-specific antibodies

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Imaging cytometry by multiparameter fluorescence

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluorescent tandem phycobiliprotein conjugates. Emission wavelength shifting by energy transfer

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

UV lasers for flow cytometric analysis: HeCd versus argon laser excitation

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

THE EFFECT OF HYDROGEN ION CONCENTRATION ON FLUORESCENT LABELLED ANTIBODIES

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Approaches to multicolor immunofluorescence measurements

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

A versatile means of intracellular labeling: injection of biocytin and its detection with avidin conjugates

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Species-specific second antibodies reduce spurious staining in immunocytochemistry

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

A 38,000-dalton membrane protein (p38) present in synaptic vesicles

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

A simple method of reducing the fading of immunofluorescence during microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Aminomethyl coumarin acetic acid: a new fluorescent labelling agent for proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

A biotin-containing compound N-(2-aminoethyl)biotinamide for intracellular labeling and neuronal tracing studies: Comparison with biocytin

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

The use of phycobiliproteins as fluorescent labels in immunoassay

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Spatial and dynamic changes in intracellular Ca2+ measured by confocal laser-scanning microscopy in bullfrog sympathetic ganglion cells

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluorescence emission spectra of plant leaves and plant constituents

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Single laser three color immunofluorescence staining procedures based on energy transfer between phycoerythrin and cyanine 5.

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Molecular mechanisms of intracellular calcium excitability in X. laevis oocytes

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Two-color immunofluorescence using a fluorescence-activated cell sorter

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Modulation of hexokinase association with mitochondria analyzed with quantitative three-dimensional confocal microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Improvements for the anatomical characterization of insect neurons in whole mount: the use of cyanine-derived fluorophores and laser scanning confocal microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Computerized quantification of immunofluorescence-labeled axon terminals and analysis of co-localization of neurochemicals in axon terminals with a confocal scanning laser microscope

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluorescent phycobiliprotein conjugates for analyses of cells and molecules

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

The laser-scanning confocal microscope in biomedical research

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

A novel fluorescent ceramide analogue for studying membrane traffic in animal cells: accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Strategic location of calcium channels at transmitter release sites of frog neuromuscular synapses

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous unique properties

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Aberration compensation in confocal microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Structural and functional considerations of GABA in islets of Langerhans. Beta-cells and nerves

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Cyanine dye labeling reagents--carboxymethylindocyanine succinimidyl esters

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Hydrophobic adsorption chromatography to reduce nonspecific staining by rhodamine-labeled antibodies

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Three-color immunofluorescence histochemistry allowing triple labeling within a single section

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Confocal microscopy as a tool for the study of the intranuclear topography of chromosomes.

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Analysis by confocal scanning laser microscopy imaging of the spatial distribution of intermediate filaments in foetal and adult rat liver cells.

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

H+/base transport in principal cells characterized by confocal fluorescence imaging

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Fluoro-Gold: composition, and mechanism of uptake

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Which fluorophore is brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, Texas red, and cyanine 3.18

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

A method for immunofluorescent demonstration of three coexisting neurotransmitters in rat brain and spinal cord, using the fluorophores fluorescein, lissamine rhodamine, and 7-amino-4-methylcoumarin-3-acetic acid

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

A spectrophotometric method for determination of fluorophore-to-protein ratios in conjugates of the blue fluorophore 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Detection and characterization of a sensory microganglion associated with the spinal accessory nerve: A scanning laser confocal microscopic study of the neurons and their processes

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Cascade Blue derivatives: Water soluble, reactive, Blue emission dyes evaluated as fluorescent labels and tracers

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Spectral and photophysical studies of benzo[c]xanthene dyes: dual emission pH sensors

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Photostability studies of phycobiliprotein fluorescent labels

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Sensitive detection of RNAs in single cells by flow cytometry

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2802%2970006-X

7 January 2021

Confocal scanning optical microscopy and its applications for biological specimens

1 reference

COCI

https://opencitations.net/index/api/v1/citations/10.1242/JCS.94.2.175

10.1016/S0091-679X(02)70006-X

10 June 2024

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12512325%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

1 reference