Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries. (Q31967754)

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6 December 2019

title

Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries (English)

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6 December 2019

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5

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6 December 2019

author name string

J Hanes

1

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6 December 2019

L Jermutus

2

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6 December 2019

S Weber-Bornhauser

3

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H R Bosshard

4

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6 December 2019

language of work or name

English

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publication date

1 November 1998

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Proceedings of the National Academy of Sciences of the United States of America

6 December 2019

published in

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6 December 2019

volume

95

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6 December 2019

issue

24

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6 December 2019

page(s)

14130-14135

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Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library

6 December 2019

cites work

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Molecular evolution by staggered extension process (StEP) in vitro recombination

21 June 2018

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Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites

21 June 2018

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RNA-peptide fusions for the in vitro selection of peptides and proteins

21 June 2018

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21 June 2018

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In vitro selection and evolution of functional proteins by using ribosome display

21 June 2018

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High-affinity peptide ligands to prostate-specific antigen identified by polysome selection

21 June 2018

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Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

21 June 2018

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Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site

21 June 2018

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Minimizing a binding domain from protein A.

21 June 2018

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Rapid evolution of a protein in vitro by DNA shuffling

21 June 2018

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21 June 2018

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Protein-protein interactions: methods for detection and analysis

21 June 2018

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Diversity of oligonucleotide functions

21 June 2018

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An in vitro polysome display system for identifying ligands from very large peptide libraries

21 June 2018

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CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range

21 June 2018

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In vitro selection of bacteriophage Q-beta ribonucleic acid variants resistant to ethidium bromide.

21 June 2018

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Lambda chains and genes in inbred mice

21 June 2018

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GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast

21 June 2018

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The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins

21 June 2018

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Calculation of protein extinction coefficients from amino acid sequence data

21 June 2018

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Fidelity of DNA polymerases in DNA amplification

21 June 2018

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SELEXION. Systematic evolution of ligands by exponential enrichment with integrated optimization by non-linear analysis

21 June 2018

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Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli

21 June 2018

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In vitro virus: bonding of mRNA bearing puromycin at the 3'-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro

21 June 2018

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Crossref

https://api.crossref.org/works/10.1073%2FPNAS.95.24.14130

Real-time competitive kinetic analysis of interactions between low-molecular-weight ligands in solution and surface-immobilized receptors

21 January 2018

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Competition BIAcore for measuring true affinities: large differences from values determined from binding kinetics.

21 January 2018

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https://api.crossref.org/works/10.1073%2FPNAS.95.24.14130

Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions

21 January 2018

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Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay

31 October 2018

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12 December 2020

In vitro transcription: preparative RNA yields in analytical scale reactions

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12 December 2020

Spectroscopic, calorimetric, and kinetic demonstration of conformational adaptation in peptide-antibody recognition

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12 December 2020

Mimicking somatic hypermutation: affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain

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12 December 2020

Identifiers

DOI

10.1073/PNAS.95.24.14130

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:9826665%20AND%20SRC:MED&resulttype=core&format=json

6 December 2019

0 references

1 reference

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6 December 2019

PubMed publication ID

9826665

1 reference