Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes (Q33444742)

26 July 2017

title

Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes (English)

1 reference

26 July 2017

author

Sylvestre Marillonnet

series ordinal

4

object named as

Sylvestre Marillonnet

1 reference

26 July 2017

Carola Engler

1

1 reference

26 July 2017

Ramona Gruetzner

series ordinal

2

1 reference

26 July 2017

Romy Kandzia

series ordinal

3

1 reference

26 July 2017

language of work or name

English

0 references

publication date

14 May 2009

1 reference

26 July 2017

1 reference

26 July 2017

volume

4

1 reference

26 July 2017

issue

5

1 reference

26 July 2017

page(s)

e5553

1 reference

Creative Commons Attribution 4.0 International

26 July 2017

copyright license

start time

14 May 2009

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

0 references

cites work

Sequence-independent construction of ordered combinatorial libraries with predefined crossover points

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

A one pot, one step, precision cloning method with high throughput capability

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Combinatorial recombination of gene fragments to construct a library of chimeras

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Developments in directed evolution for improving enzyme functions

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Biocatalytic conversion of avermectin to 4''-oxo-avermectin: improvement of cytochrome p450 monooxygenase specificity by directed evolution.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Mutant library construction in directed molecular evolution: casting a wider net.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Multivalent avimer proteins evolved by exon shuffling of a family of human receptor domains

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Novel methods for directed evolution of enzymes: quality, not quantity

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

General method for sequence-independent site-directed chimeragenesis

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Growth factor engineering by degenerate homoduplex gene family recombination

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable alpha-amylase

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Libraries of hybrid proteins from distantly related sequences

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

DNA shuffling method for generating highly recombined genes and evolved enzymes

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

A combinatorial approach to hybrid enzymes independent of DNA homology

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Molecular evolution by staggered extension process (StEP) in vitro recombination

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

DNA shuffling of a family of genes from diverse species accelerates directed evolution

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Rapid evolution of a protein in vitro by DNA shuffling

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

Randomization of genes by PCR mutagenesis

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2677662

A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme

14 July 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0005553

A general one-step method for the cloning of PCR products.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0005553

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0005553

Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0005553

Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/19436741

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1371/JOURNAL.PONE.0005553

1 reference

26 July 2017

0 references

1 reference

26 July 2017

1 reference