Electrophoretic methods for process monitoring and the quality assessment of recombinant glycoproteins (Q77665512)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:9848665%20AND%20SRC:MED&resulttype=core&format=json

7 December 2019

review article

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title

Electrophoretic methods for process monitoring and the quality assessment of recombinant glycoproteins (English)

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7 December 2019

1

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7 December 2019

author name string

Tran NT

2

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7 December 2019

Merry T

3

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7 December 2019

Horvath E

4

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7 December 2019

Ferrier D

5

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7 December 2019

publication date

1 November 1998

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7 December 2019

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7 December 2019

volume

19

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7 December 2019

issue

15

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7 December 2019

page(s)

2572-2594

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The oligosaccharides of glycoproteins: bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties

7 December 2019

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Comparison of ampholytes used for slab gel and capillary isoelectric focusing of recombinant tissue-type plasminogen activator glycoforms

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Prediction of the in vivo biological activity of human recombinant follicle stimulating hormone using quantitative isoelectric focusing

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Influence of cell- and media-derived factors on the integrity of a human monoclonal antibody after secretion into serum-free cell culture supernatants

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Capillary electrophoretic separation of human recombinant erythropoietin (r-HuEPO) glycoforms

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Rapid one-step capillary isoelectric focusing method to monitor charged glycoforms of recombinant human tissue-type plasminogen activator

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Capillary isoelectric focusing and sodium dodecyl sulfate-capillary gel electrophoresis of recombinant humanized monoclonal antibody HER2

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Sequence verification of human creatine kinase (43 kDa) isozymes by high-resolution tandem mass spectrometry

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High-yield expression and purification of recombinant human macrophage migration inhibitory factor.

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Molecular cloning of the cowpea leghemoglobin II gene and expression of its cDNA in Escherichia coli. Purification and characterization of the recombinant protein

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A recombinant human hemoglobin with asparagine-102(beta) substituted by alanine has a limiting low oxygen affinity, reduced marginally by chloride

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Isoelectric focusing of Tamm-Horsfall glycoproteins: a simple tool for recognizing recurrent calcium oxalate renal stone formers

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7 January 2021

Transferrin microheterogeneity as a probe in normal and disease states

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7 January 2021

Protein purification in multicompartment electrolyzers with isoelectric membranes

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7 January 2021

Characterization of proteins by sequential isoelectric focusing on immobilized pH gradients and electrospray mass spectrometry

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7 January 2021

Investigation on minor degraded derivatives of the recombinant hirudin variant HM2 from Hirudinaria manillensis isolated by isoelectric focusing in multicompartment electrolyzers.

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7 January 2021

Purification of human recombinant superoxide dismutase by isoelectric focusing in a multicompartment electrolyzer with zwitterionic membranes.

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Isolation of Escherichia coli synthesized recombinant eukaryotic proteins that contain epsilon-N-acetyllysine

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7 January 2021

Sequence-specific deamidation: isolation and biochemical characterization of succinimide intermediates of recombinant hirudin.

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7 January 2021

Purification of recombinant human growth hormone by isoelectric focusing in a multicompartment electrolyzer with Immobiline membranes.

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7 January 2021

Analysis of recombinant proteins by isoelectric focusing in immobilized pH gradients

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7 January 2021

Sample streaks and smears in immobilized pH gradient gels

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7 January 2021

Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension

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7 January 2021

Characterization of changes in the glycosylation pattern of recombinant proteins from BHK-21 cells due to different culture conditions

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7 January 2021

Enhanced secretion of human granulocyte colony-stimulating factor directed by a novel hybrid fusion peptide from recombinant Saccharomyces cerevisiae at high cell concentration

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7 January 2021

Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography

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7 January 2021

Two-dimensional electrophoresis as a tool for control of quality and consistency in production systems using animal cells. Two-dimensional electrophoresis in animal cell culture technology

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7 January 2021

Baculovirus-Mediated Expression of Chicken Growth Hormone

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7 January 2021

High-resolution separation of recombinant human interferon-gamma glycoforms by micellar electrokinetic capillary chromatography

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Analysis of Serum Proteins by Micellar Electrokinetic Capillary Chromatography. Application to a Drug Carrier Evaluation

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7 January 2021

High-performance capillary electrophoresis of glycoproteins: the use of modifiers of electroosmotic flow for analysis of microheterogeneity

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7 January 2021

Capillary zone electrophoresis and packed capillary column liquid chromatographic analysis of recombinant human interleukin-4

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7 January 2021

High-resolution glycoprotein analysis using capillary electrophoresis

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7 January 2021

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7 January 2021

Effect of polyamines on the separation of ovalbumin glycoforms by capillary electrophoresis

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7 January 2021

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7 January 2021

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7 January 2021

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7 January 2021

Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis. Effects of pH, buffer type and organic additives

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7 January 2021

Conformational diversity and conformational transitions of a monoclonal antibody monitored by circular dichroism and capillary electrophoresis

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7 January 2021

Incorporation of hydrophobic selectivity in capillary electrophoresis: analysis of recombinant insulin-like growth factor I variants

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7 January 2021

Control of the cultivation process of antithrombin III and its characterization by capillary electrophoresis

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7 January 2021

Method of analysis of recombinant acidic fibroblast growth factor by capillary electrophoresis

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7 January 2021

Profiling of oligosaccharide-mediated microheterogeneity of a monoclonal antibody by capillary electrophoresis

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7 January 2021

Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis

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7 January 2021

Sodium dodecyl sulfate-polymer capillary electrophoresis for the analysis of cell culture proteins

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7 January 2021

Analysis of recombinant human erythropoietin in drug formulations by high-performance capillary electrophoresis

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7 January 2021

Analysis of a recombinant granulocyte macrophage colony stimulating factor dosage form by capillary electrophoresis, capillary isoelectric focusing and high-performance liquid chromatography

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7 January 2021

Differences between natural and recombinant interleukin-2 revealed by gel electrophoresis and capillary electrophoresis

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7 January 2021

Separation of recombinant human growth hormone from Escherichia coli cell pellet extract by capillary zone electrophoresis

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7 January 2021

Examination of capillary zone electrophoresis, capillary isoelectric focusing and sodium dodecyl sulfate capillary electrophoresis for the analysis of recombinant tissue plasminogen activator

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7 January 2021

Analysis of recombinant DNA-derived glycoproteins via high-performance capillary electrophoresis coupled with off-line matrix-assisted laser desorption ionization time-of-flight mass spectrometry

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7 January 2021

Direct isoform analysis of high-mannose-containing glycoproteins by on-line capillary electrophoresis electrospray mass spectrometry

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7 January 2021

Capillary isoelectric focusing: the problem of protein solubility

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7 January 2021

Determination of the isoelectric points of low and high molecular mass ampholytes by capillary electrophoresis.

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7 January 2021

Capillary isoelectric focusing

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7 January 2021

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7 January 2021

Determination of pI by measuring the current in the mobilization step of high-performance capillary isoelectric focusing

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7 January 2021

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7 January 2021

Quantitation of glycated hemoglobins in human adult blood by capillary isoelectric focusing

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7 January 2021

Effects of catholytes on the mobilization of proteins after capillary isoelectric focusing

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7 January 2021

Effects of electroosmotic flow on zone mobilization in capillary isoelectric focusing

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7 January 2021

Solubilization of proteins for electrophoretic analyses

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7 January 2021

Capillary isoelectric focusing of the scrapie prion protein

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7 January 2021

Current trends in capillary isoelectric focusing of proteins

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7 January 2021

Capillary electrophoresis in biotechnology

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7 January 2021

Capillary isoelectric focusing of proteins

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7 January 2021

Capillary isoelectric focusing as a tool in the examination of antibodies, peptides and proteins of pharmaceutical interest

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7 January 2021

Electrophoretic separation of recombinant tissue-type plasminogen activator glycoforms: validation issues for capillary isoelectric focusing methods

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7 January 2021

Non-native capillary isoelectric focusing for the analysis of the microheterogeneity of glycoproteins

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7 January 2021

Validation of a capillary isoelectric focusing method for the recombinant monoclonal antibody C2B8

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7 January 2021

Rapid high-performance isoelectric focusing of monoclonal antibodies in uncoated fused-silica capillaries

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7 January 2021

High-performance capillary electrophoresis for in-process control in the production of antithrombin III and human clotting factor IX

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7 January 2021

Capillary isoelectric focusing-electrospray ionization mass spectrometry for transferrin glycoforms analysis

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7 January 2021

High-performance sodium dodecyl sulfate polyacrylamide gel capillary electrophoresis of peptides and proteins

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7 January 2021

High-performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks

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7 January 2021

Sodium dodecyl sulfate-capillary gel electrophoresis of proteins using non-cross-linked polyacrylamide

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7 January 2021

Sodium dodecyl sulfate polyacrylamide gel- and replaceable polymer-filled capillary electrophoresis for molecular mass determination of proteins of pharmaceutical interest

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7 January 2021

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7 January 2021

Automated Ferguson analysis of glycoproteins by capillary electrophoresis using a replaceable sieving matrix

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7 January 2021

A new type of pH- and detergent-stable coating for elimination of electroendosmosis and adsorption in (capillary) electrophoresis

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7 January 2021

Recent advances in capillary electrophoresis of carbohydrates

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7 January 2021

Capillary electrophoresis of carbohydrates

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7 January 2021

Separation of neutral carbohydrates by capillary electrophoresis

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7 January 2021

Detection of carbohydrates in capillary electrophoresis

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7 January 2021

Simultaneous determination of reducing monosaccharides by capillary zone electrophoresis as the borate complexes of N-2-pyridylglycamines

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7 January 2021

Analysis of the oligosaccharides in ovalbumin by high-performance capillary electrophoresis

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7 January 2021

Capillary zone electrophoresis of linear and branched oligosaccharides

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7 January 2021

Ultrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis

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7 January 2021

High-performance capillary electrophoresis of unsaturated oligosaccharides derived from glycosaminoglycans by digestion with chondroitinase ABC as 1-phenyl-3-methyl-5-pyrazolone derivatives

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7 January 2021

High resolution and rapid analysis of branched oligosaccharides by capillary electrophoresis

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7 January 2021

Capillary zone electrophoresis of oligosaccharides derivatized with various aminonaphthalene sulfonic acids

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7 January 2021

High-resolution capillary gel electrophoresis of reducing oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonate

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7 January 2021

Separation of 1-aminopyrene-3,6,8-trisulfonate-labeled asparagine-linked fetuin glycans by capillary gel electrophoresis

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7 January 2021

N-glycosylation site mapping of recombinant tissue plasminogen activator by micellar electrokinetic capillary chromatography

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Investigation of micelles and anionic cyclodextrins as pseudostationary phases for the capillary electrophoresis separation of oligosaccharides derivatized with 2-amino-benzamide

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Capillary electrophoretic examination of underivatized O-linked and N-linked oligosaccharide mixtures and immunoglobulin G antibody-released oligosaccharide libraries

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7 January 2021

Capillary and slab gel electrophoresis profiling of oligosaccharides

1 reference

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7 January 2021

High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Fluorophore-assisted carbohydrate electrophoresis technology and applications.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Characterization of the glycosylation of a human IgM produced by a human-mouse hybridoma.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

A method for monitoring the glycosylation of recombinant glycoproteins from conditioned medium, using fluorophore-assisted carbohydrate electrophoresis

1 reference

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7 January 2021

The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. Detection of picomolar quantities by an imaging system based on a cool

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Fluorophore-labelled carbohydrate electrophoresis distinguishes rheumatoid arthritis from healthy individuals

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Capillary electrophoresis of proteins under alkaline conditions

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Method optimization in capillary zone electrophoretic analysis of hGH tryptic digest fragments

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Evaluation of glycosylation site heterogeneity and selective identification of glycopeptides in proteolytic digests of bovine alpha 1-acid glycoprotein by mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

The effect of phytic acid on the resolution of peptides and proteins in capillary electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Improved peptide mapping using phytic acid as ion-pairing buffer additive in capillary electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Capillary zone electrophoresis of alpha 1-acid glycoprotein fragments from trypsin and endoglycosidase digestions

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Peptide mapping and evaluation of glycopeptide microheterogeneity derived from endoproteinase digestion of erythropoietin by affinity high-performance capillary electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Combination of capillary electrophoresis and matrix-assisted laser desorption ionization mass spectrometry for glycosylation analysis of a human monoclonal anti-Rhesus(D) antibody.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Generation of tryptic maps of alpha- and beta-globin chains by capillary electrophoresis in isoelectric buffers

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Capillary zone electrophoresis of peptide fragments from trypsin digestion of biosynthetic human growth hormone

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Comparison of the utility of capillary zone electrophoresis and high-performance liquid chromatography in peptide mapping and separation

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Application of capillary electrophoresis, high-performance liquid chromatography, on-line electrospray mass spectrometry and matrix-assisted laser desorption ionization--time of flight mass spectrometry to the characterization of single-chain plasmi

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Peptide mapping using combinations of size-exclusion chromatography, reversed-phase chromatography and capillary electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

The use of sequential high-performance liquid chromatography and capillary zone electrophoresis to separate the glycosylated peptides from recombinant tissue plasminogen activator to a detailed level of microheterogeneity

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Multiple sequential fraction collection of peptides and glycopeptides by high-performance capillary electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Recent advances in capillary electrophoresis/electrospray/mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Sheathless capillary electrophoresis-electrospray ionization mass spectrometry using 10 μm I.D. capillaries: Analyses of tryptic digests of cytochrome c

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

On-line capillary zone electrophoresis-ion spray tandem mass spectrometry for the determination of dynorphins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Peptide mapping of complex proteins at the low-picomole level with capillary electrophoretic separations

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Characterization of glycopeptides from recombinant coagulation factor VIIa by high-performance liquid chromatography and capillary zone electrophoresis using ultraviolet and pulsed electrochemical detection

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Capillary electrophoresis with pulsed amperometric detection of carbohydrates and glycopeptides

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Development of electrophoretic conditions for the characterization of protein glycoforms by capillary electrophoresis-electrospray mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Coupling capillary zone electrophoresis and continuous-flow fast atom bombardment mass spectrometry for the analysis of peptide mixtures

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Use of an ion trap storage/reflectron time-of-flight mass spectrometer as a rapid and sensitive detector for capillary electrophoresis in protein digest analysis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Capillary electrophoresis-atmospheric pressure ionization mass spectrometry for the characterization of peptides. Instrumental considerations for mass spectrometric detection

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Recent advances in liquid chromatography-mass spectrometry and capillary zone electrophoresis-mass spectrometry for protein analysis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Protein identification by capillary zone electrophoresis/microelectrospray ionization-tandem mass spectrometry at the subfemtomole level

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Use of a Polybrene Capillary Coating in Capillary Electrophoresis for Rapid Analysis of Hemoglobin Variants with On-Line Detection via an Ion Trap Storage/Reflectron Time-of-Flight Mass Spectrometer

1 reference

https://api.crossref.org/works/10.1002%2FELPS.1150191505

7 January 2021

Identifiers

DOI

10.1002/ELPS.1150191505

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:9848665%20AND%20SRC:MED&resulttype=core&format=json

7 December 2019

1 reference