Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens (Q33654299)

28 July 2017

title

Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens (English)

1 reference

28 July 2017

0 references

Oana Ursu

4

0 references

Evan A Boyle

object named as

Evan A Boyle

3

0 references

Anshul Kundaje

object named as

Anshul Kundaje

14

0 references

William Greenleaf

13

object named as

William J Greenleaf

1 reference

28 July 2017

David W Morgens

1

1 reference

28 July 2017

Michael Wainberg

2

1 reference

28 July 2017

Carlos L Araya

5

1 reference

28 July 2017

C Kimberly Tsui

6

1 reference

28 July 2017

Michael S Haney

7

1 reference

28 July 2017

Gaelen T Hess

8

1 reference

28 July 2017

Kyuho Han

9

1 reference

28 July 2017

Edwin E Jeng

10

1 reference

28 July 2017

Amy Li

11

1 reference

28 July 2017

Michael P Snyder

12

1 reference

28 July 2017

Michael C Bassik

15

1 reference

28 July 2017

language of work or name

English

0 references

publication date

5 May 2017

1 reference

28 July 2017

published in

Nature Communications

1 reference

28 July 2017

volume

8

1 reference

28 July 2017

page(s)

15178

1 reference

Creative Commons Attribution 4.0 International

28 July 2017

copyright license

start time

5 May 2017

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

0 references

cites work

Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Rationally engineered Cas9 nucleases with improved specificity

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Identification and characterization of essential genes in the human genome.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Paradoxical resistance of multiple myeloma to proteasome inhibitors by decreased levels of 19S proteasomal subunits

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Next-generation libraries for robust RNA interference-based genome-wide screens.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

High-throughput functional genomics using CRISPR-Cas9.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Integrative analysis of 111 reference human epigenomes

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Improved vectors and genome-wide libraries for CRISPR screening

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Measuring error rates in genomic perturbation screens: gold standards for human functional genomics

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Genome-scale CRISPR-Cas9 knockout screening in human cells

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Genetic screens in human cells using the CRISPR-Cas9 system

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Systematic discovery and characterization of regulatory motifs in ENCODE TF binding experiments

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

GENCODE: the reference human genome annotation for The ENCODE Project

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

An integrated encyclopedia of DNA elements in the human genome

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

A human embryonic kidney 293T cell line mutated at the Golgi alpha-mannosidase II locus

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Fast and accurate short read alignment with Burrows-Wheeler transform

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Profiling essential genes in human mammary cells by multiplex RNAi screening

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

3' UTR seed matches, but not overall identity, are associated with RNAi off-targets

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Expression profiling reveals off-target gene regulation by RNAi

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5424143

Molecular biology. Use and abuse of RNAi to study mammalian gene function

14 July 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNCOMMS15178

Microhomology-based choice of Cas9 nuclease target sites.

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNCOMMS15178

CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNCOMMS15178

21 January 2018

Identifiers

DOI

10.1038/NCOMMS15178

1 reference

Dimensions Publication ID

28 July 2017

0 references

0 references

1 reference

28 July 2017

1 reference